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  • © 2015

Plant Genotyping

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1245)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. Advances in Plant Genotyping: Where the Future Will Take Us

    • Dhwani A. Patel, Manuel Zander, Jessica Dalton-Morgan, Jacqueline Batley
    Pages 1-11
  3. Molecular Marker Applications in Plants

    • Alice C. Hayward, Reece Tollenaere, Jessica Dalton-Morgan, Jacqueline Batley
    Pages 13-27
  4. Molecular Marker Databases

    • Kaitao Lai, MichaÅ‚ Tadeusz Lorenc, David Edwards
    Pages 49-62
  5. SSR Genotyping

    • Annaliese S. Mason
    Pages 77-89
  6. Genotyping Analysis Using an RFLP Assay

    • Shutao Dai, Yan Long
    Pages 91-99
  7. DNA Barcoding for Plants

    • Natasha de Vere, Tim C. G. Rich, Sarah A. Trinder, Charlotte Long
    Pages 101-118
  8. Multiplexed Digital Gene Expression Analysis for Genetical Genomics in Large Plant Populations

    • Christian Obermeier, Bertha M. Salazar-Colqui, Viola Spamer, Rod Snowdon
    Pages 119-140
  9. SNP Genotyping by Heteroduplex Analysis

    • Norma Paniego, Corina Fusari, Verónica Lia, Andrea Puebla
    Pages 141-150
  10. Challenges of Genotyping Polyploid Species

    • Annaliese S. Mason
    Pages 161-168
  11. Genomic Reduction Assisted Single Nucleotide Polymorphism Discovery Using 454-Pyrosequencing

    • Peter J. Maughan, Joshua A. Udall, Eric N. Jellen
    Pages 169-182
  12. Inter-SINE Amplified Polymorphism (ISAP) for Rapid and Robust Plant Genotyping

    • Torsten Wenke, Kathrin M. Seibt, Thomas Döbel, Katja Muders, Thomas Schmidt
    Pages 183-192
  13. Screening of Mutations by TILLING in Plants

    • Nian Wang, Lei Shi
    Pages 193-203
  14. SNP Genotyping Using KASPar Assays

    • Scott M. Smith, Peter J. Maughan
    Pages 243-256
  15. Skim-Based Genotyping by Sequencing

    • Agnieszka A. Golicz, Philipp E. Bayer, David Edwards
    Pages 257-270

About this book

The ability to produce vast amounts of DNA sequence data has enabled the discovery of molecular markers in model organisms, crops, as well as orphan species making genotyping the rate limiting factor, and this volume focuses on the different markers available and the low to high throughput genotyping of these markers. Given the diverse nature of some of these systems, an overview is provided on the identification of markers from sequence data, as well as data analysis with example applications once the genotyping data has been generated. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 Authoritative and easily accessible, Plant Genotyping: Methods and Protocols is aimed at plant molecular biologists, geneticists, plant breeders and ecologists who have a target question and need to know the most suitable markers and genotyping system to use.

Editors and Affiliations

  • School of Plant Biology, The University of Western Australia, Crawley, Australia

    Jacqueline Batley

Bibliographic Information

  • Book Title: Plant Genotyping

  • Book Subtitle: Methods and Protocols

  • Editors: Jacqueline Batley

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-1966-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-1965-9Published: 06 November 2014

  • Softcover ISBN: 978-1-4939-4367-8Published: 23 August 2016

  • eBook ISBN: 978-1-4939-1966-6Published: 05 November 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 315

  • Number of Illustrations: 21 b/w illustrations, 34 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access