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New Techniques of Optical Microscopy and Microspectroscopy

  • Textbook
  • © 1991

Overview

Editors:
  1. Richard J. Cherry

    1. Dept of Chemistry and Biological Chemistry, University of Essex, Colchester, England

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Part of the book series: Topics in Molecular and Structural Biology (TMSB)

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Table of contents (10 chapters)

  1. Front Matter

    Pages i-xii
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  2. Video and Opto-Digital Imaging Microscopy

    • David M. Shotton
    Pages 1-47

  3. The Confluence of Advances in Light Microscopy: CCD, Confocal, Near-field and Molecular Exciton Microscopy

    • Aaron Lewis
    Pages 49-89

  4. Quantitative Interference Microscopy

    • Graham A. Dunn
    Pages 91-118

  5. The Dynamic Study of Cell Surface Organization by Nanoparticle Video Microscopy

    • H. Geerts, M. De Brabander, R. Nuydens, R. Nuyens
    Pages 119-136

  6. Microscope Laser Light Scattering Spectroscopy

    • Joyce A. Peetermans, Izumi Nishio, Toyoichi Tanaka
    Pages 137-152

  7. Differential Polarization Microscopy

    • William E. Mickols
    Pages 153-176

  8. Time-resolved Fluorescence Microscopy

    • Rujiang Tian, Michael A. J. Rodgers
    Pages 177-198

  9. Fluorescence Photobleaching Techniques

    • Reiner Peters, Manfred Scholz
    Pages 199-228

  10. Measuring Cytoplasmic Calcium in Single Living Cells Using Fluorescent Probes

    • Ron Jacob, Christopher D. Benham
    Pages 229-253

  11. Membrane Potential Imaging

    • Leslie M. Loew
    Pages 255-272

  12. Back Matter

    Pages 273-279
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Keywords

About this book

The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The development of techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutionising the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sensor molecules for cellular assays. This volume brings together these various advances in order to provide the reader with an up to date account of what can now be achieved with modern optical microscopic methods.

Editors and Affiliations

  • Dept of Chemistry and Biological Chemistry, University of Essex, Colchester, England

    Richard J. Cherry

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