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Gene Transfer and Expression

A Laboratory Manual

  • Book
  • © 1990

Overview

Authors:
  1. Michael Kriegler
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Table of contents (12 chapters)

  1. Front Matter

    Pages i-xiii
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  2. Gene Transfer

    1. Front Matter

      Pages 1-1
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    2. Eukaryotic Control Elements

      • Michael Kriegler
      Pages 3-22

    3. Vectors

      • Michael Kriegler
      Pages 23-61

    4. Processing of Proteins Encoded by Transferred Genes

      • Michael Kriegler
      Pages 62-65

  3. Gene Transfer Methods

    1. Front Matter

      Pages 83-83
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    2. Cells and Cell Lines

      • Michael Kriegler
      Pages 85-95

    3. DNA Transfer

      • Michael Kriegler
      Pages 96-102

    4. Selection and Amplification

      • Michael Kriegler
      Pages 103-113

    5. Expression Cloning

      • Michael Kriegler
      Pages 114-135

    6. Subtractive Hybridization

      • Michael Kriegler
      Pages 136-160

    7. Retrovirus-Mediated Gene Transfer

      • Michael Kriegler
      Pages 161-164

    8. PCR-Based Expression

      • Michael Kriegler
      Pages 165-172

  4. Assays for Gene Transfer and Expression

    1. Front Matter

      Pages 177-177
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    2. Assays for Gene Transfer

      • Michael Kriegler
      Pages 179-204

    3. Assays for Gene Expression

      • Michael Kriegler
      Pages 205-226

  5. Back Matter

    Pages 229-242
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Keywords

About this book

A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.

Bibliographic Information

  • Book Title: Gene Transfer and Expression

  • Book Subtitle: A Laboratory Manual

  • Authors: Michael Kriegler

  • DOI: https://doi.org/10.1007/978-1-349-11891-5

  • Publisher: Palgrave Macmillan London

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Stockton Press 1990

  • Softcover ISBN: 978-1-349-11893-9Published: 01 January 1990

  • eBook ISBN: 978-1-349-11891-5Published: 18 June 1990

  • Edition Number: 1

  • Number of Pages: XIII, 242

  • Topics: Biochemistry, general

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