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Foodborne Bacterial Pathogens

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Arnaud Bridier

    1. Fougères Laboratory, ANSES, Fougères, France

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  • Includes cutting-edge method and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1918)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
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  2. Bacterial Pathogen Detection and Quantification in Food

    1. Front Matter

      Pages 1-1
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    2. Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products

      • Eduardo Ximenes, Seockmo Ku, Lori Hoagland, Michael R. Ladisch
      Pages 3-20

    3. Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens

      • Maggie R. Williams, Syed A. Hashsham
      Pages 21-33

    4. The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens

      • Alejandro Garrido-Maestu, David Tomás Fornés, Marta Prado Rodríguez
      Pages 35-45

    5. Sequence-Specific End Labeling of Oligonucleotides (SSELO)-Based Microbial Detection

      • Tanja Kostic, Levente Bodrossy
      Pages 47-56

    6. Rapid On-Site Detection and Quantification of Foodborne Pathogens Using Microfluidic Devices

      • Nobuyasu Yamaguchi
      Pages 57-66

    7. Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria

      • Matthew Dunne, Martin J. Loessner
      Pages 67-86

    8. EIS-Based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes

      • Palmiro Poltronieri, Elisabetta Primiceri, Rajeswaran Radhakrishnan
      Pages 87-101

  3. Phenotypic and Metabolic Characterization of Foodborne Pathogens

    1. Front Matter

      Pages 103-103
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    2. Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions

      • Olivier Habimana
      Pages 105-116

    3. Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopic Observation

      • Thomas Brauge, Graziella Midelet-Bourdin, Christophe Soumet
      Pages 117-128

    4. Isolation of Bacterial RNA from Foods Inoculated with Pathogens

      • Deepti Tyagi, Autumn L. Kraft, Teresa M. Bergholz
      Pages 129-137

    5. Use of Two-Dimensional Electrophoresis to Explore Foodborne Bacterial Pathogen Responses to Gastrointestinal Stress

      • Denise Schrama, Maria Leonor Faleiro
      Pages 139-147

    6. Identification of Putative Biomarkers Specific to Foodborne Pathogens Using Metabolomics

      • Snehal R. Jadhav, Rohan M. Shah, Avinash V. Karpe, David J. Beale, Konstantinos A. Kouremenos, Enzo A. Palombo
      Pages 149-164

    7. Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells

      • Florence Dubois-Brissonnet
      Pages 165-170

    8. High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Structural Dynamic of Foodborne Pathogens

      • Alexis Canette, Julien Deschamps, Romain Briandet
      Pages 171-182

    9. Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae

      • Macarena A. Varas, Javiera Ortíz-Severín, Andrés E. Marcoleta, Carlos A. Santiviago, Miguel L. Allende, Francisco P. Chávez
      Pages 183-190

    10. Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection

      • Francisco P. Chávez, Andrea Calixto
      Pages 191-197

  4. NGS and Modeling Approaches

    1. Front Matter

      Pages 199-199
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Keywords

About this book

This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students,  and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens.


Editors and Affiliations

  • Fougères Laboratory, ANSES, Fougères, France

    Arnaud Bridier

Bibliographic Information

  • Book Title: Foodborne Bacterial Pathogens

  • Book Subtitle: Methods and Protocols

  • Editors: Arnaud Bridier

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9000-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8999-7Published: 23 December 2018

  • eBook ISBN: 978-1-4939-9000-9Published: 22 December 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 278

  • Number of Illustrations: 10 b/w illustrations, 43 illustrations in colour

  • Topics: Microbiology, Food Science

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