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T-Follicular Helper Cells

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Luis Graca

    1. Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible result
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2380)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Identification of Tfh and Tfr Cells in Humans and Mice

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Complete Visualization of T Follicular Helper Cells in Germinal Centers by Light Sheet Fluorescence Microscopy

      • Liat Stoler-Barak, Ziv Shulman
      Pages 3-13

    3. In Vivo Imaging of Tfh Cells

      • Sandra Nakandakari-Higa, Johanne T. Jacobsen
      Pages 15-27

    4. Circulating T Follicular Helper Subsets in Human Blood

      • Yayoi Iwamoto, Hideki Ueno
      Pages 29-39

    5. Identification of Human Blood and Tissue T Follicular Regulatory (Tfr) Cells by Flow Cytometry

      • Filipa Ribeiro, Pedro Ávila-Ribeiro, João Eurico Fonseca, Luis Graca
      Pages 41-46

    6. Using Mass Cytometry to Address Tfh and Tfr Heterogeneity

      • James B. Wing, Shimon Sakaguchi
      Pages 47-57

    7. Identification of T Peripheral Helper (Tph) Cells

      • Vanessa S. Wacleche, Runci Wang, Deepak A. Rao
      Pages 59-76

    8. Identification of Non-classical Follicular T Cells

      • Alan Courey-Ghaouzi, Mauro Gaya
      Pages 77-84

    9. Identification of Follicular T Cells in the Gut

      • Hristo Georgiev, Georgia Papadogianni, Günter Bernhardt
      Pages 85-95

  3. Development and Function of Tfh and Tfr Cells

    1. Front Matter

      Pages 97-97
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    2. In Vivo Migration and Tfh Cell Interactions

      • Bo Liu, Hai Qi
      Pages 99-109

    3. A Method to Analyze Models of the Dynamics of Germinal Centers

      • José Faro, Emilio Faro
      Pages 111-123

    4. Identification of Antigen Presenting Cell Subsets Supporting Human Tfh Differentiation

      • Lamine Alaoui, Mélanie Durand, Elodie Segura
      Pages 125-139

    5. The Use of Peptide-MHCII Tetramers to Identify Antigen-Specific T Follicular Helper and T Follicular Regulatory Cells

      • Meryem Aloulou, Nicolas Fazilleau
      Pages 141-147

    6. Assessment of the TCR Repertoire of Human Circulating T Follicular Helper Cells

      • Mengyun Hu, Federica Sallusto
      Pages 149-163

    7. Assessing In Vivo T Cell-Dependent Antigen-Specific Antibody Responses

      • Markus M. Xie, Alexander L. Dent
      Pages 165-174

    8. Tfh-Mediated and Tfr-Suppressed Antigen-Driven IgG and IgE Assays

      • Rachel L. Clement, Benjamin L. Hanson, Peter T. Sage
      Pages 175-185

  4. Tfh and Tfr Cells in Infectious and Inflammatory Diseases

    1. Front Matter

      Pages 187-187
      Download chapter PDF

    2. Assessment of the Impact of Cytokines on TFH, TREG, and TFR Cell Populations After Influenza Infection

      • Amber Papillion, Meagan M. Jenkins, André Ballesteros-Tato
      Pages 189-199
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Keywords

About this book

The volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical models to study GC dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, T-Follicular Helper Cells: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Editors and Affiliations

  • Instituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal

    Luis Graca

Bibliographic Information

  • Book Title: T-Follicular Helper Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Luis Graca

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1736-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1735-9Published: 22 November 2021

  • Softcover ISBN: 978-1-0716-1738-0Published: 23 November 2022

  • eBook ISBN: 978-1-0716-1736-6Published: 21 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 294

  • Number of Illustrations: 18 b/w illustrations, 51 illustrations in colour

  • Topics: Immunology

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