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Plant Cell Division

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2382)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Mitosis and Cell Cycle Progression

    1. Front Matter

      Pages 17-17
    2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana

      • Ulkar Ahmadli, Michael Sandmann, Joerg Fuchs, Inna Lermontova
      Pages 19-28
    3. A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips

      • Leigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, William F. Thompson
      Pages 29-72
    4. Analysis of Commitment Point Attainment in Algae Dividing by Multiple Fission

      • Veronika Kselíková, Vilém Zachleder, Kateřina Bišová
      Pages 89-101
  3. Manipulation of Cell Division

    1. Front Matter

      Pages 103-103
    2. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement Cells

      • Tamara Jimenez-Gόngora, Huang Tan, Rosa Lozano-Durán
      Pages 115-127
  4. Quantification of Cell Division Patterning

    1. Front Matter

      Pages 129-129
    2. Kinematic Growth Analysis of Rice (Oryza sativa) Leaf

      • Jingjing Fang, Xueyong Li
      Pages 131-140
    3. Studying Cell Division Plane Positioning in Early-Stage Embryos

      • Katia Belcram, Jean-Christophe Palauqui, Martine Pastuglia
      Pages 141-154
    4. Means to Quantify Vascular Cell File Numbers in Different Tissues

      • Helena E. Arents, Gugan Eswaran, Matouš Glanc, Ari Pekka Mähönen, Bert De Rybel
      Pages 155-179
    5. Quantification of Cell Division Angles in the Arabidopsis Root

      • Katia Belcram, David Legland, Martine Pastuglia
      Pages 209-221
  5. Imaging and Quantifying Plant Cytokinesis

    1. Front Matter

      Pages 223-223
    2. A Quantitative Method for Evaluating Phragmoplast Morphology

      • Takema Sasaki, Yoshihisa Oda
      Pages 225-232

About this book

This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression.

Editors and Affiliations

  • SiCE, Laboratoire Reproduction et Développement des Plantes, ENS Lyon, Lyon, France

    Marie-Cécile Caillaud

Bibliographic Information

  • Book Title: Plant Cell Division

  • Book Subtitle: Methods and Protocols

  • Editors: Marie-Cécile Caillaud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1744-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1743-4Published: 28 October 2021

  • Softcover ISBN: 978-1-0716-1746-5Published: 29 October 2022

  • eBook ISBN: 978-1-0716-1744-1Published: 27 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 267

  • Number of Illustrations: 18 b/w illustrations, 42 illustrations in colour

  • Topics: Plant Sciences, Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access