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Single-Cell Protein Analysis

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Aik T. Ooi

    1. Mission Bio, Inc., South San Francisco, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2386)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections

    • Preethi Vijayaraj
    Pages 1-16

  3. Indirect Immunofluorescence of Tissue Sections

    • Cody J. Aros
    Pages 17-26

  4. Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique

    • Valentina Proserpio, Laura Conti, Salvatore Oliviero
    Pages 27-41

  5. High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry

    • Marco A. Fernandez, Hammad Alzayat, Maria C. Jaimes, Yacine Kharraz, Gerard Requena, Pedro Mendez
    Pages 43-60

  6. Detection of Cytokine-Secreting Cells by Enzyme-Linked Immunospot (ELISpot)

    • Bernt Axelsson
    Pages 61-79

  7. Detection and Enumeration of Cytokine-Secreting Cells by FluoroSpot

    • Bernt Axelsson
    Pages 81-99

  8. Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing

    • Samuel C. Kim, John R. Haliburton, Zev J. Gartner, Adam R. Abate
    Pages 101-111

  9. Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis

    • Ákos Végvári, Jimmy E. Rodriguez, Roman A. Zubarev
    Pages 113-127

  10. Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels

    • Carlene Petes, Stephen K. H. Li, Shariq Mujib, Michelle M. Poulin, Noah Saederup, Andrew A. Quong et al.
    Pages 129-145

  11. High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging

    • Ofer Elhanani, Leeat Keren, Michael Angelo
    Pages 147-156

  12. Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA

    • Jing Lin, Savaş Tay
    Pages 157-169

  13. High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform

    • David W. Ruff, Dalia M. Dhingra, Kathryn Thompson, Jacqueline A. Marin, Aik T. Ooi
    Pages 171-188

  14. Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform

    • Nicole Mende, Elisa Laurenti, Berthold Göttgens, Nicola K. Wilson
    Pages 189-201

  15. Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets

    • Benjamin Lacar
    Pages 203-217

  16. Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits

    • Mandi Wong, Carol Kosman, Liane Takahashi, Naveen Ramalingam
    Pages 219-261

  17. Combined Measurement of RNA and Protein Expression on a Single-Cell Level

    • Valentina Russo, Nadia Brasu, Luigia Pace
    Pages 263-288

  18. Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities

    • Dalia M. Dhingra, Aik T. Ooi, David W. Ruff
    Pages 289-307

  19. Correction to: High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry

    • Marco A. Fernandez, Hammad Alzayat, Maria C. Jaimes, Yacine Kharraz, Gerard Requena, Pedro Mendez
    Pages C1-C3

  20. Back Matter

    Pages 309-311
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Keywords

About this book

This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.

Editors and Affiliations

  • Mission Bio, Inc., South San Francisco, USA

    Aik T. Ooi

Bibliographic Information

  • Book Title: Single-Cell Protein Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Aik T. Ooi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1771-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1770-0Published: 12 November 2021

  • Softcover ISBN: 978-1-0716-1773-1Published: 13 November 2022

  • eBook ISBN: 978-1-0716-1771-7Published: 11 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 311

  • Number of Illustrations: 95 illustrations in colour

  • Topics: Chemistry/Food Science, general, Cell Biology

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