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Invariant Natural Killer T-Cells

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Chaohong Liu

    1. Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2388)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Genetic Analysis of iNKT Cell Development and Function

    • Julie Sahler, Orchi Anannya, Candice Limper, Brian Imbiakha, Tim Pierpont, Avery August
    Pages 1-12

  3. Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells

    • Dapeng Zhou, Chengfeng Xia, Peng G. Wang, Zhiyuan Li, Wenpeng Zhang, Guanghui Ni et al.
    Pages 13-25

  4. Retroviral Transduction of NKT Hybridoma Cells

    • Ke Wang, Rong Jin, Qing Ge
    Pages 27-34

  5. Methods for Studying Mouse and Human Invariant Natural Killer T Cells

    • Yang Zhou, Yan-Ruide Li, Samuel Zeng, Lili Yang
    Pages 35-57

  6. Isolation and Detection of Murine iNKT Cells in Different Organs

    • Mengqing Cong, Xiang Li, Haopeng Fang, Li Bai, Xucai Zheng, Bofeng Li
    Pages 59-69

  7. Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

    • Wenjing He, Dongmei Ye, Yifang Gao
    Pages 71-77

  8. Isolation and Characterization Methods of Human Invariant NKT Cells

    • Liu Rui, Wang Hua
    Pages 79-85

  9. Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry

    • Vibhuti Joshi, Masaki Terabe
    Pages 87-99

  10. Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry

    • Jihene Klibi, Kamel Benlagha
    Pages 101-112

  11. iNKT Cel Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach

    • Marcella Cipelli, Theresa Ramalho, Cristhiane Favero de Aguiar, Niels Olsen Saraiva Camara
    Pages 113-122

  12. Expansion of Human iNKT Cells Ex Vivo

    • Jing Wang, Chen Zhao, Jianqing Xu
    Pages 123-129

  13. The Expansion and Cytotoxicity Detection of Human iNKT Cells

    • Xue Cheng, Xiaosheng Tan, Rui Dou, Xiongwen Wu, Xiufang Weng
    Pages 131-137

  14. Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

    • Natasha K. Khatwani, Kelly J. Andrews, Asha B. Pillai
    Pages 139-148

  15. Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice

    • Zhou Hong, Zeng Zhutian, Wang Fei
    Pages 149-156

  16. In Vivo Cytotoxicity by α-GalCer-transactivated NK Cells

    • Patrick T. Rudak, S. M. Mansour Haeryfar
    Pages 157-174

  17. Redirecting iNKT Cell Antitumor Immunity with α-GalCer/CD1d-scFv Fusion Proteins

    • Lianjun Zhang, Alena Donda
    Pages 175-180

  18. Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles During Development

    • Jana L. Raynor, Hongbo Chi
    Pages 181-192

  19. Back Matter

    Pages 193-195
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Keywords

About this book

This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.

Editors and Affiliations

  • Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Chaohong Liu

Bibliographic Information

  • Book Title: Invariant Natural Killer T-Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Chaohong Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1775-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1774-8Published: 16 September 2021

  • Softcover ISBN: 978-1-0716-1777-9Published: 16 September 2022

  • eBook ISBN: 978-1-0716-1775-5Published: 15 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 195

  • Number of Illustrations: 13 b/w illustrations, 33 illustrations in colour

  • Topics: Immunology

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