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Neural Progenitor Cells

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Loic P. Deleyrolle

    1. Department of Neurosurgery, University of Florida, Gainesville, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2389)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Culturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition

    • Hassan Azari
    Pages 1-10

  3. Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region

    • Alessandra Ricca, Federica Cascino, Angela Gritti
    Pages 11-31

  4. Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres

    • Ruslan Rust, Tara L. Walker
    Pages 33-44

  5. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord

    • Jean-Philippe Hugnot
    Pages 45-56

  6. Culturing and Expansion of “ Clinical Grade ” Neural Stem Cells from the Fetal Human Central Nervous System

    • Maurizio Gelati, Daniela Celeste Profico, Daniela Ferrari, Angelo Luigi Vescovi
    Pages 57-66

  7. Microglia Isolation from Neural Stem Cell-Enriched Regions

    • Gregory P. Marshall II
    Pages 67-75

  8. Microglial FACS for Robust RNA Recovery for Next-Generation Sequencing

    • Andria L. Doty, Aline Oliveria
    Pages 77-86

  9. Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded Under Neurosphere Culture

    • Nasser Nassiri Koopaei, Thomas D. Schmittgen, Brent A. Reynolds, Hassan Azari
    Pages 87-94

  10. Isolating and Culturing of Precursor Cells from the Adult Human Brain

    • Florian A. Siebzehnrubl
    Pages 95-102

  11. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

    • Luc Bauchet, Gaetan Poulen, Nicolas Lonjon, Florence Vachiery-Lahaye, Emmanuel Bourinet, Florence Evelyne Perrin et al.
    Pages 103-110

  12. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny

    • Hassan Azari
    Pages 111-123

  13. Identifying Neural Progenitor Cells in the Adult Human Brain

    • Thomas I. H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L. M. Faull et al.
    Pages 125-154

  14. Neonatal Transplant in Hypoxic Injury

    • Tong Zheng, Michael D. Weiss
    Pages 155-164

  15. Isolation and Purification of Self-Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke

    • Marcel M. Daadi
    Pages 165-175

  16. Generating Cerebral Organoids from Human Pluripotent Stem Cells

    • Leon Chew, Adam Añonuevo, Erin Knock
    Pages 177-199

  17. Advancing Our Understanding of Brain Disorders : Research Using Postmortem Brain Tissue

    • Maurice A. Curtis, Vinata Vedam-Mai
    Pages 201-208

  18. Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases

    • Vinata Vedam-Mai
    Pages 209-220

  19. Back Matter

    Pages 221-223
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Keywords

About this book

This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.

Editors and Affiliations

  • Department of Neurosurgery, University of Florida, Gainesville, USA

    Loic P. Deleyrolle

Bibliographic Information

  • Book Title: Neural Progenitor Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Loic P. Deleyrolle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1783-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1782-3Published: 24 September 2021

  • Softcover ISBN: 978-1-0716-1785-4Published: 25 September 2022

  • eBook ISBN: 978-1-0716-1783-0Published: 23 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 223

  • Number of Illustrations: 14 b/w illustrations, 41 illustrations in colour

  • Topics: Neurosciences, Stem Cells, Anatomy

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