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PCR Primer Design

  • Book
  • © 2022

Overview

Editors:
  1. Chhandak Basu

    1. Department of Biology, California State University, Northridge, Los Angeles, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible result
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2392)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Primer Design for Genotyping

    1. Front Matter

      Pages 1-1
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    2. The Significance of PCR Primer Design in Genetic Diversity Studies: Exemplified by Recent Research into the Genetic Structure of Marine Species

      • Madjid Delghandi, Marit Pedersen Delghandi, Stephen Goddard
      Pages 3-15

    3. Enhancing Cohort PASA Efficiency from Lessons Assimilated by Mutant Genotyping in C. elegans

      • Amita Pandey, Binu Bhat, Madan L. Aggarwal, Girdhar K. Pandey
      Pages 17-33

    4. Design of Oligonucleotides for Allele-Specific Amplification Based on PCR and Isothermal Techniques

      • Luis Antonio Tortajada-Genaro
      Pages 35-51

    5. Detection of Rubella Virus by Tri-Primer RT-PCR Assay and Genotyping by Fragment RT-PCR

      • Suji George
      Pages 53-64

    6. Design of Mismatch Primers to Identify and Differentiate Closely Related (Sub)Species: Application to the Authentication of Meat Products

      • Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
      Pages 65-82

    7. Primer Design for the Analysis of Closely Related Species: Application of Noncoding mtDNA and cpDNA Sequences

      • Lidia Skuza
      Pages 83-91

    8. Designing PCR Primers for the Amplification-Refractory Mutation System

      • Majid Komijani, Khashayar Shahin, Esam Ibraheem Azhar, Mohammad Bahram
      Pages 93-99

  3. Primer Design for Genome-Wide Identification of Specific Regions

    1. Front Matter

      Pages 101-101
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    2. Validation of Circular RNAs by PCR

      • Aniruddha Das, Debojyoti Das, Amaresh C. Panda
      Pages 103-114

    3. Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana

      • Pinky Dhatterwal, Sandhya Mehrotra, Rajesh Mehrotra
      Pages 115-123

  4. Primer Design for Multiplex PCR. Multiplex

    1. Front Matter

      Pages 125-125
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    2. PLASmid TAXonomic PCR (PlasTax-PCR), a Multiplex Relaxase MOB Typing to Assort Plasmids into Taxonomic Units

      • Raquel Cuartas, Teresa M. Coque, Fernando de la Cruz, M. Pilar Garcillán-Barcia
      Pages 127-142

    3. Multiplex PCR Design for Scalable Resequencing

      • Darren Korbie, Matt Trau
      Pages 143-158

  5. Primer Design for qPCR

    1. Front Matter

      Pages 159-159
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    2. Identification of Gene Copy Number in the Transgenic Plants by Quantitative Polymerase Chain Reaction (qPCR)

      • Poonam Kanwar, Soma Ghosh, Sibaji K. Sanyal, Girdhar K. Pandey
      Pages 161-171

    3. qPrimerDB: A Powerful and User-Friendly Database for qPCR Primer Design

      • Wei Chang, Yue Niu, Mengna Yu, Tian Li, Jiana Li, Kun Lu
      Pages 173-182

  6. Primer Design for Identification of Plant and Animal Viruses

    1. Front Matter

      Pages 183-183
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    2. PCR Primer Design for the Rapidly Evolving SARS-CoV-2 Genome

      • Wubin Qu, Jiangyu Li, Haoyang Cai, Dongsheng Zhao
      Pages 185-197
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Keywords

About this book

This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.

Editors and Affiliations

  • Department of Biology, California State University, Northridge, Los Angeles, USA

    Chhandak Basu

Bibliographic Information

  • Book Title: PCR Primer Design

  • Editors: Chhandak Basu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1799-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1798-4Published: 14 November 2021

  • Softcover ISBN: 978-1-0716-1801-1Published: 14 November 2022

  • eBook ISBN: 978-1-0716-1799-1Published: 13 November 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIII, 276

  • Number of Illustrations: 90 b/w illustrations

  • Topics: Human Genetics

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